德国默克全系列 *产品
酵母菌杂交
一、实验原理
酵母菌的生活史属于双单性类型，它们的单倍体细胞和二倍体细胞一般都能进行无限制的分裂。以单倍体细胞为主的酵母菌称为单倍体酵母，例如粟酒裂殖酵母（Schizosaccharamycespombe）。以二倍体细胞为主的酵母菌称为二倍体酵母，如啤酒酵母（又称面包酵母，Saccharomycescerevisiae）。这两种酵母常用作遗传学研究的材料，而啤酒酵母在酿酒工业和食品工业中又有很大的应用价值。本实验用异宗配合型的啤酒酵母的单倍体菌株进行杂交实验。任何一株酵母菌，如果使二倍体的营养细胞处于形成孢子的条件下，都会发生减数分裂，生成四分体和子囊。每个子囊孢子具有四分子核，正常情况下子囊中有4个子囊孢子，其中有2个a和2个α，a和α表示两种相对的接合型。具有这两种接合型的子囊孢子萌发生长成单倍体的菌株，不同接合型的单倍体菌株细胞接合生成二倍体菌株。具有这样生活史的酵母菌称为异宗配合型酵母。异宗配合型啤酒醇母的生活史见图11－1。
单倍体菌株分属a和α两种接合型，是受一对等位基因a和α控制的，这对等位基因的座位在第三连锁群靠近着丝粒的地方。在光学显微镜下观察酵母的染色体是十分困难的，但借助于电子显微镜，对酵母菌的细胞学已了解得较多了。现已知啤酒酵母具有17个连锁群（n=17）。
两个属于不同接合型的营养缺陷型的酵母单倍体菌株，在基本培养基上都不能形成菌落。当它们杂交形成二倍体杂种细胞，就能在基本培养基上生长。二倍体杂种细胞在形成子囊孢子过程中，发生染色体重组，而使产生的子囊孢子出现重组性状。
二、实验目的
通过酵母菌杂交实验了解异宗配合型啤酒酵母的生活史和基因自由组合定律，掌握酵母菌杂交的基本方法。
三、实验材料
1.啤酒酵母单倍体腺嘌呤缺陷型（ade-），接合型为a；
2.啤酒酵母单倍体组氨酸缺陷型（Hisl-），接合型为α。
四、实验器具和药品
1.用具：试管，离心管，离心机，吸管，培养皿，三角烧瓶，涂布棒，石英砂。
2.药品：生理盐水（0.85克NaCl溶于100毫升蒸馏水中，15磅压力下消毒15分钟），纤维素酶或蜗牛酶。
3.培养基：
（1）*液体培养基：蛋白胨2克，酵母浸母汁1克，葡萄糖2克，水100毫升，pH6.0，8磅压力下灭菌30分钟。
（2）*固体培养基：在*液体培养基中加2％琼脂。
（3）基本液体培养基：葡萄糖10克，（NH4）2SO41克，K2HPO40.125克，KH2PO40.875克，KI*母液1毫升，MgSO4·7H2O0.5克，CaCl2·2H2O0.1克，NaCl0.1克，微量元素母液**1毫升，维生素母液***1毫升，水1000毫升，pH5.3，8磅压力下灭菌30分钟。
*KI母液：10毫克KI于100毫升水中。
**微量元素母液：H3PO41毫克，ZnSO4·7H2O7毫克，CuSO4·5H2O1毫克，CoCl2·6H2O5毫克，水100毫升。
***维生素母液：烟碱酸40毫克，维生素B140毫克，肌醇200毫克，核黄素20毫克，对-氨基苯甲酸20毫克，吡哆醇40毫克，泛酸40毫克，生物素0.2毫克，水100毫升。
（4）基本固体培养基：在基本液体培养基中加2％琼脂。
（5）产孢子培养基：CH3COONa8.2克，KCl1.86克，吡哆酵母液*1毫升，泛酸母液**1毫升，生物素母液***1毫升，琼脂20克，蒸馏水1000毫升。 德国默克全系列 *产品 BPHHSP-BASE Widescreen? BeadPlex? Human Heat Shock Protein Panel -Base
BPHHSP-H27 Human Heat Shock Protein 27
BPHHSP-H60 Heat Shock Protein 60 (HSPD1)
BPHHSP-H70 Heat Shock Protein 70 (HSP72, HSPA1A&A1B
BPHHSP-H90 Heat Shock Protein 90 alpha (HSPAA1)
BPHHSP-P27 Phospho-Heat Shock Protein 27 (Ser78/Ser82)
03-32-0009-100MG ELASTASE SUBSTRATE VIII, COLORIMETRIC
03-32-1501-25MG Z-PHE-ARG-7-AMIDO-4-METHYLCOUMARIN, HCL
03-32-1501-5MG Z-PHE-ARG-7-AMIDO-4-METHYLCOUMARIN, HCL
03-32-5032-1MG MMP-2/MMP-7 SUBSTRATE, FLUOROGENIC
03-32-5033-1MG MMP-2/MMP-7 SUBSTRATE CONTROL
03-34-0001-25MG H-GLY-PRO-ARG-PRO-OH
03-34-0001-5MG H-GLY-PRO-ARG-PRO-OH
03-34-0002-25MG H-ARG-GLY-ASP-SER-OH
03-34-0027-25MG H-GLY-ARG-GLY-ASP-SER-OH
03-34-0035-25MG H-GLY-ARG-GLY-ASP-SER-PRO-OH
03-34-0035-5MG H-GLY-ARG-GLY-ASP-SER-PRO-OH
03-34-0051-100MG CALPEPTIN
03-34-0051-25MG CALPEPTIN
03-34-0051-5MG CALPEPTIN
03-34-0052-25MG H-GLY-ARG-ALA-ASP-SER-PRO-OH
03-34-0052-5MG H-GLY-ARG-ALA-ASP-SER-PRO-OH
03-34-0055-25MG H-GLY-ARG-GLY-ASP-THR-PRO-OH
03-34-0055-5MG H-GLY-ARG-GLY-ASP-THR-PRO-OH
05-22-0930-100GM B-ENDORPHIN (HUMAN)
05-22-2500-25MG FORMYL-MET-LEU-PHE-OH
05-22-3838-1MG BQ-788, SODIUM SALT
05-22-3838-5MG BQ-788, SODIUM SALT
05-23-0050-0.1MG CORTICOTROPIN-REL. FACTOR,HUMAN+RAT
05-23-0050-1MG CORTICOTROPIN-REL. FACTOR,HUMAN+RAT
05-23-0070-1MG A-HELICAL CORTICOTROPIN-REL. FACTOR 9-41
05-23-0101-1MG ANGIOTENSIN II, HUMAN
05-23-0101-25MG ANGIOTENSIN II, HUMAN
05-23-0101-5MG ANGIOTENSIN II, HUMAN
05-23-0111-5MG [TYR(PO3H2)4]-ANGIOTENSIN II, HUMAN
05-23-0150-1MG [ARG8]-VASOPRESSIN
05-23-0150-5MG [ARG8]-VASOPRESSIN
05-23-0300-0.5MG ATRIAL NATRIURETIC FACTOR 1-28, HUMAN
05-23-0300-1MG ATRIAL NATRIURETIC FACTOR 1-28, HUMAN
05-23-0310-0.5MG NATRIURETIC PEPTIDE C-TYPE HUMAN+PORCINE
05-23-0310-1MG NATRIURETIC PEPTIDE C-TYPE HUMAN+PORCINE
05-23-0500-1MG BRADYKININ
05-23-0500-25MG BRADYKININ
05-23-0500-5MG BRADYKININ
05-23-0600-1MG SUBSTANCE P
05-23-0600-25MG SUBSTANCE P
05-23-0600-5MG SUBSTANCE P
05-23-0613-1MG SENKTIDE
05-23-0701-1MG NEUROKININ A
05-23-0751-1MG A-MELANOCYTE-STIMULATING HORMONE
05-23-0751-5MG A-MELANOCYTE-STIMULATING HORMONE
05-23-0753-1MG ADRENOCORTICOTROPIC HORMONE 1-24, HUMAN
05-23-0756-5MG [NLE4,D-PHE7]-A-MELANOCYTE-STIM. HORMONE
05-23-0800-1MG DYNORPHIN A 1-13, PORCINE
05-23-0850-1MG SOMATOSTATIN
05-23-0850-5MG SOMATOSTATIN
05-23-0930-1MG B-ENDORPHIN, HUMAN
05-23-1200-5MG NEUROTENSIN
05-23-1701-1MG FR-1
05-23-2005-0.5MG NEUROPEPTIDE Y, HUMAN
05-23-2005-1MG NEUROPEPTIDE Y, HUMAN
05-23-2101-0.5MG VASOACTIVE INTESTINAL PEP HUMAN+PORC+RAT
05-23-2101-1MG VASOACTIVE INTESTINAL PEP HUMAN+PORC+RAT
05-23-2102-1MG SECRETIN, HUMAN
05-23-2150-0.1MG PACAP 38, OVINE
05-23-2150-1MG PACAP 38, OVINE
05-23-2151-0.5MG PACAP 27 AMIDE, OVINE
05-23-2301-1MG GASTRIN I, HUMAN
05-23-2401-25MG CALCITONIN, SALMON
05-23-2402-0.5MG CALCITONIN, HUMAN
05-23-2402-1MG CALCITONIN, HUMAN
05-23-2404-0.5MG CALCITONIN GENE-RELATED PEPTIDE-I, HUMAN
05-23-2404-1MG CALCITONIN GENE-RELATED PEPTIDE-I, HUMAN
05-23-2540-0.5MG AMYLIN, HUMAN
05-23-2700-0.1MG GLUCAGON, HUMAN
05-23-2700-0.5MG GLUCAGON, HUMAN
05-23-3703-1MG LAMININ NONAPEPTIDE
05-23-3703-5MG LAMININ NONAPEPTIDE
05-23-3800-0.1MG ENDOTHELIN 1, HUMAN AND PORCINE
05-23-3800-0.5MG ENDOTHELIN 1, HUMAN AND PORCINE
05-23-3800-1MG ENDOTHELIN 1, HUMAN AND PORCINE
05-23-3801-0.1MG ENDOTHELIN 3, HUMAN AND RAT
05-23-3801-0.5MG ENDOTHELIN 3, HUMAN AND RAT
05-23-3813-.1MG SARAFOTOXIN S6C ATRACTASPSIS ENGADDENSIS
05-23-3813-.5MG SARAFOTOXIN S6C ATRACTASPSIS ENGADDENSIS
05-23-3831-1MG BQ-123, SODIUM SALT
05-23-3831-5MG BQ-123, SODIUM SALT
05-23-4904-1MG PROTEIN KINASE C INHIBITOR PEPTIDE 19-31
05-23-4904-5MG PROTEIN KINASE C INHIBITOR PEPTIDE 19-31
05-23-4910-5MG SYNTIDE 2
05-23-5501-1MG PARATHYROID HORMONE 1-34, HUMAN
100050-5MG 1400W
100064-50UG 14-3-3ZETA, GST-FUSION, HU., RECOM
100065-100MG 2-APB
100066-25MG 5HPP-33
100068-500UG 17-AAG
100069-500UG 17-DMAG
100071-100UG 14-3-3BETA, GST-FUSION, HUMAN, REC.
100072-100UG 14-3-3GAMMA, GST-FUSION, HUMAN, REC.
100105-10MG A23187, FREE ACID, S. CHARTREUSENSIS
100105-1MG A23187, FREE ACID, S. CHARTREUSENSIS
100105-50MG A23187, FREE ACID, S. CHARTREUSENSIS
100105-5MG A23187, FREE ACID, S. CHARTREUSENSIS
100106-10MG A23187, MIXED CALCIUM-MAGNESIUM SALT
100107-1MG A23187, 4-BROMO-
100109-10MG AACOCF3
100110-1GM AAPH
100122-10MG A3, HCL
100128-5MG A77 1726
101500-100MG AEBSF, HCL
101500-1GM AEBSF, HCL
101500-500MG AEBSF, HCL
101500-50MG AEBSF, HCL
102555-2000U ABL PROTEIN TYROSINE KINASE, RECOMB.
104550-25MG ACA
104850-1SET ACTIN POLYMERIZATION INTERFER AGENTS SET
1063-100MG ACETYLCOENZYME A, TRILITHIUM SALT
1063-10MG ACETYLCOENZYME A, TRILITHIUM SALT
106425-5GM N-ACETYL-L-CYSTEINE
107920-200UG ANTI-ACRP30 RABBIT PAB
1079-25GM N-ACETYL-A-D-GLUCOSAMINE
108975-100MG LEUPEPTIN, HEMISULFATE
108975-10MG LEUPEPTIN, HEMISULFATE
108975-25MG LEUPEPTIN, HEMISULFATE
108975-50MG LEUPEPTIN, HEMISULFATE
108975-5MG LEUPEPTIN, HEMISULFATE
108976-10MG LEUPEPTIN, HEMISULFATE, SYNTHETIC
108976-25MG LEUPEPTIN, HEMISULFATE, SYNTHETIC
110116-1U B1-2,3,4,6-N-AC-GLUCOSAMINIDASE, RECOM
110123-5MG ACETYL-11-KETO-B-BOSWELLIC ACID
110138-1GM N-ACETYLNEURAMINIC ACID, SYNTHETIC
110145-5MG D-ERYTHRO-SPHINGOSINE, N-ACETYL-
110150-5MG N-AC-D-GLUCOSAMINYL-B1,4-N-AC-MURAMYL-L-
110153-2MG N-ACETYLGALACTOSAMINE A1-O-SERINE
110175-1MG ACETYL-PEPSTATIN
112251-1MG A1-ACID GLYCOPROTEIN, HUMAN SERUM
113000-1GM ACRIDINE ORANGE
113200-500UL ANTI-A-ACTIN MOUSE MAB (1A4)
114666-1SET ACTINOMYCIN D, STREPTOMYCES SP.
114666-5MG ACTINOMYCIN D, STREPTOMYCES SP.
114700-5UG ACTIVIN A, HU., RECOM, CHO CELL LINE
114711-25UG FOLLISTATIN 300, HU., RECOM
114798-25MG ACYCLOVIR
114802-250UG ADAAHX3L3VS
114810-5MG ADAMTS-5 INHIBITOR
1152-100GM ADENINE
1152-10GM ADENINE
1152-25GM ADENINE
115500-50UG ADENOPHOSTIN A, HEXASODIUM SALT
1160-100GM ADENOSINE
1160-10GM ADENOSINE
116530-50MG N(6),O(2')-DIOCTANOYL-CAMP, SODIUM SALT
116801-100MG ADENOSINE-3'5'-CYCLIC MONOPHOSPHATE, NA
116801-1GM ADENOSINE-3'5'-CYCLIC MONOPHOSPHATE, NA
116802-10UMOL N(6)-BENZOYL-CAMP, SODIUM SALT
116805-500UG PROTEIN KINASE A INHIBITOR 5-24
116811-1KIT CAMP DIRECT IMMUNOASSAY KIT
116812-10MG 8-(4-CHLOROPHENYLTHIO)-CAMP, SODIUM SALT
116814-5UMOL CAMPS, RP-ISOMER, TEA SALT
116815-10UMOL CAMPS, SP-ISOMER, TEA SALT
116816-5UMOL CAMPS, 8-BROMO-, RP-ISOMER, SODIUM SALT
116818-5UMOL CAMPS, 8-BROMO-, SP-ISOMER, SODIUM SALT
116819-5UMOL ADENOSINE 3,5-CYCLIC PHOSPHOTHIOATE 8-CL
116820-1ST ANTI-CAMP RABBIT PAB
116825-5UMOL ADENOSINE 3,5-CYCLIC PHOSPHOTHIOATE,2'-O
116833-5UMOL 8-CPT-2'-O-ME-CAMP
116850-25MG ADENYLYL CYCLASE TYPE V INHIBITOR, NKY80
116850-5MG ADENYLYL CYCLASE TYPE V INHIBITOR, NKY80
116880-500U ADENOSINE DEAMINASE, BOVINE INTESTINE
116890-5MG ADENOSINE KINASE INHIBITOR
117105-1GM ADENOSINE 5'-DIPHOSPHATE, POTASSIUM SALT
117110-1MG ADP-RIBOSYLATION FACTOR 6, N-TERM., MYR
1171-1GM ADENOSINE 5'-DIPHOSPHATE, MONOSODIUM
1171-5GM ADENOSINE 5'-DIPHOSPHATE, MONOSODIUM
118110-5GM ADENSOINE 5'-MONOPHOSPHATE, DISOD. SALT
118410-10MG ADENOSINE 3'-PHOSPHATE 5'-PHOSPHOSULFAT
118415-1SET ADP-HPD, DIHYDRATE, AMMONIUM
118415-60UG ADP-HPD, DIHYDRATE, AMMONIUM
118500-2MG ATM KINASE INHIBITOR *KU-55933*
118501-5MG ATM/ATR KINASE INHIBITOR *CGK773*
118502-2MG INSOLUTION ATM KINASE INHIBITOR
119101-50UL ANTI-H+,K+-ATPASE A-SUB (1019-34) RB PAB
119102-50UL ANTI-H+,K+-ATPASE, A-SUB (1-17) RB PAB
119107-1KIT ATP ASSAY KIT
119109-1EA LUCIFERIN-LUCIFERASE
119113-5MG LUCIFERASE INHIBITOR I
119114-5MG LUCIFERASE INHIBITOR II
119117-100UL ANTI-A1AR (309-326) RABBIT PAB
1191-1GM ADENOSINE 5'-TRIPHOSPHATE, DISODIUM SALT
119120-25MG ATP-G-S, TETRALITHIUM SALT
119123-100UL ATP-G-S, SOLUTION, TETRALITHIUM SALT
119125-1GM ADENOSINE 5'-TRIPHOSPHATE, DISODIUM
1191-25GM ADENOSINE 5'-TRIPHOSPHATE, DISODIUM SALT
119127-1SET CAGED ATP, DISODIUM SALT
119127-5MG CAGED ATP, DISODIUM SALT
119134-25MG ADENOSINE 5'-O-(2-THIODIPHOSPHATE), 3LI
1191-50GM ADENOSINE 5'-TRIPHOSPHATE, DISODIUM SALT
1191-5GM ADENOSINE 5'-TRIPHOSPHATE, DISODIUM SALT
119186-100U ATP SULFURYLASE, S. CEREVISIAE
120002-25MG ADENYLYL-IMIDODIPHOSPHATE, TETRALITHIUM
121000-1EA Adipogenesis Inducers Panel
121739-25GM ADONITOL
121745-1MG 4-AF DA
121765-2MG AG 825
121767-1MG AG 1024
12177-1KG AGAR
121800-10MG ADVANCED GLYCATION ENDPRODUCT-BSA
121852-100GM AGAROSE, TYPE C
121853-100GM AGAROSE, TYPE I, MOLECULAR BIOLOGY GRADE
121853-500GM AGAROSE, TYPE I, MOLECULAR BIOLOGY GRADE
121937-2ML IMMOBILIZED ANTI-MOUSE IGG
121975-5UG W-AGATOXIN IVA, AGELENOPSIS APERTA
122117-5MG LECTIN, PEANUT, ARACHIS HYPOGAEA
122302-5UG W-AGATOXIN TK, AGELENOPSIS APERTA
123040-50MG AICA-RIBOSIDE
123041-25MG AICA-RIBOSIDE, 5'-PHOSPHATE
123900-1MG AKTIDE-2T
124001-10T PD ANTI-AKT1 (PTHR308) RABBIT PAB
124002-100UL ANTI-AKT2 RABBIT PAB
124003-1SET PD ANTI-AKT1 (PSER473) MOUSE MAB (11E6)
124005-1MG AKT INHIBITOR
124006-20UG AKT1, HIS-TAG, ACTIVATED, HUMAN, RECOMB.
124007-1KIT AKT ACTIVITY IMMUNOASSAY KIT
124008-1MG AKT INHIBITOR II
124009-1MG AKT INHIBITOR III
124011-1MG AKT INHIBITOR IV
124011-5MG AKT INHIBITOR IV
124012-1MG AKT INHIBITOR V, TRICIRIBINE
124013-2MG AKT INHIBITOR VI, AKT-IN
124014-2MG AKT INHIBITOR VII, TAT-AKT-IN
124015-1MG INSOLUTION(TM) AKT INHIBITOR IV
124017-1MG INSOLUTION AKT INHIBITOR VIII
124018-1MG AKT INHIBITOR VIII, ISOZYME-SELECTIVE
124018-5MG AKT INHIBITOR VIII, ISOZYME-SELECTIVE
124019-5MG AKT INHIBITOR IX, API-59CJ-OME
124020-5MG AKT INHIBITOR X
124021-20UG AKT2, GST-FUSION PROTEIN, HU., RECOM
124022-5UG AKT3, GST-FUSION PROTEIN HU., RECOM
124023-5UG DDR2, GST-FUSION, HUMAN, REC.
124024-10UG TIE2, HIS*TAG, HUMAN, REC.
124026-50UG PCAF HISTONE ACETYL TRANSFERASE, RECOM
124027-1MG MYELIN BASIC PROTEIN, MOUSE
124028-5MG AKT INHIBITOR XI
124029-2MG AKTINHIBITORXII,ISOZYME-SELECTIVE,AKTI-2
124030-2MG AKTINHIBITORXIII,ISOZYME-SELECTIVE,AKTI2
124031-1EA INHIBITORSELECTAKT/PI 3-K/MTORSIGNALINGP
1250-100GM L-ALANINE
125510-5MG DIPEPTIDYLPEPTIDASE IV SUB. I, FLUOR.
12657-1GM BSA, FRACTION V, CRYSTALLINE
12657-25GM BSA, FRACTION V, CRYSTALLINE
126575-100GM BSA, FRACTION V, FATTY ACID-FREE
126575-10GM BSA, FRACTION V, FATTY ACID-FREE
12657-5GM BSA, FRACTION V, CRYSTALLINE
126579-100GM BSA, FRAC. V, FATTY ACID-POOR, EF
126579-10GM BSA, FRAC. V, FATTY ACID-POOR, EF
126584-1ML ANTI-ALBUMIN RABBIT PAB
12659-100GM ALBUMIN, BOVINE SERUM, LOW HEAVY METALS
12659-1KG ALBUMIN, BOVINE SERUM, LOW HEAVY METALS
12659-250GM ALBUMIN, BOVINE SERUM, LOW HEAVY METALS
12659-25GM ALBUMIN, BOVINE SERUM, LOW HEAVY METALS
126593-100GM BSA, FRACTION V, RIA AND ELISA GR.
126593-10GM BSA, FRACTION V, RIA AND ELISA GR.
126593-25GM BSA, FRACTION V, RIA AND ELISA GR.
12659-500GM ALBUMIN, BOVINE SERUM, LOW HEAVY METALS
12660-100GM BSA, FRACTION V, MODIFIED COHN, PH 5.2
12660-1KG BSA, FRACTION V, MODIFIED COHN, PH 5.2
126609-100GM BSA, FRAC. V, FATTY ACID, NUC, PROT-FREE
126609-10GM BSA, FRAC. V, FATTY ACID, NUC, PROT-FREE
126609-5GM BSA, FRAC. V, FATTY ACID, NUC, PROT-FREE
126615-25ML BSA, 10% AQUEOUS SOLN, NUCLEASE-FREE
126621-50ML BSA, COHN FRACTION, 30% AQUEOUS SOLN
126625-50ML BSA, 30% AQUEOUS SOLN, STABILIZER-FREE
126626-50ML BSA, 30% AQUEOUS SOLN, PRESERVATIVE-FREE
126654-1GM ALBUMIN, HUMAN SERUM, NON-DENATURED
126658-1GM HSA, FRACTION V, HIGH PURITY
126658-5GM HSA, FRACTION V, HIGH PURITY
12666-100GM HSA, FRACTION V, LOW HEAVY METALS
12666-10GM HSA, FRACTION V, LOW HEAVY METALS
12666-1GM HSA, FRACTION V, LOW HEAVY METALS
126674-25MG ALBUMIN, MOUSE
12667-50ML HSA, 30% AQUEOUS SOLN
12668-100GM ALBUMIN, HUMAN SERUM, FRACTION V
12668-10GM ALBUMIN, HUMAN SERUM, FRACTION V
126705-1ML ANTI-ALBUMIN RABBIT PAB
126722-100MG ALBUMIN, RAT
126741-500ML ALCALASE ENZYME, BACILLUS LICHENIFORMIS
126850-2.5MG A1-PDX, HUMAN, RECOMBINANT, E. COLI
126855-100MG ALENDRONATE, SODIUM SALT
126870-1MG ALSTERPAULLONE
126871-1MG ALSTERPAULLONE, 2-CYANOETHYL
126920-25MG ALDH2 AGONIST, ALDA-1
126925-100U ALDEHYDE DEHYDROGENASE, YEAST
127100-10MG ALLOPREGNANOLONE
128125-5MG ALOISINE A
128135-5MG ALOISINE, RP106
129741-1MG A-AMANITIN, AMANITA SP. |
